A Central Hub of Crispr based Genome Editing
CrisprID | Crispr2577 |
Organism | Danio rerio |
Gene/Locus (Literature) | gata5 |
NCBI Gene Name | gata5 |
Target Sequence | GACTGTCGAATAAGAGAAACCGGGCGGTGG |
Target+Mutant_Flanking | GACTGTCGAATAAGAGAAACCGGGCGGTGGATGAGGGC |
Modification Length | 31 |
Genetic Modification | insertion |
Location | Exon2 |
Conc. (ng/μl) | 20/300 |
Cell line | Embryo |
Method/Assay | SSA recombination assay |
Crispr efficiency | 50%-90% |
PRM/crRNA/Vector | U6/PXT7 |
PRM-cas/vector | CMV/pUC19 |
Pubmed | 23528705 |