CrisprGE


     A Central Hub of Crispr based Genome Editing



Crispr2604 details


CrisprIDCrispr2604
OrganismDanio rerio
Gene/Locus (Literature)fh1
NCBI Gene Namefh
Target SequenceCCCCGGTCGCCATGTACCGCTCC
Target+Mutant_Flanking

GAGAGCAGTAGTTCCGCCCCCGGTCGCCATGTACCGCTCCGCTCGCTCCCTGCATCGCTT
GAGAGCAGTAGTTCCGCCCCCG
ccc - CCATGTACCGCTCCGCTCGCTCCCTGCATCGCTT

Modification LengthD4,+3
Genetic ModificationIndels
LocationNA
Conc. (ng/μl) 36.2/100
Cell line Embryo
Method/AssayT7E1 assay
Crispr efficiency 59.40%
PRM/crRNA/VectorT7/pDR274
PRM-cas/vectorpMJ806
Pubmed23360964