CrisprGE


     A Central Hub of Crispr based Genome Editing



Crispr2629 details


CrisprIDCrispr2629
OrganismDanio rerio
Gene/Locus (Literature)fh2
NCBI Gene Namefh
Target SequenceCCATGTACCGCTCCGCTCGCTCC
Target+Mutant_Flanking

AGTTCCGCCCCCGGTCGCCATGTACCGCTCCGCTCGCTCCCTGCATCGCTTCAGCGCGAG
AGTTCCGCCCCCGGTCGCCAT
tccgcccccggtcgCCGCTCCGCTCGCTCCCTGCATCGC 

Modification LengthD3,+14
Genetic ModificationIndels
LocationNA
Conc. (ng/μl) 12.5/300
Cell line Embryo
Method/AssayT7E1 assay
Crispr efficiency 35.60%
PRM/crRNA/VectorT7/pDR274
PRM-cas/vectorpMJ806
Pubmed23360964