A Central Hub of Crispr based Genome Editing
CrisprID | Crispr2679 |
Organism | Danio rerio |
Gene/Locus (Literature) | tph1a |
NCBI Gene Name | tph1a |
Target Sequence | CCGGGCTGCGGTTGTGTTTTCCC |
Target+Mutant_Flanking | GAGTCTTCAGAGACAGGCCGGGCTGCGGTTGTGTTTTCCCTGAAAAATGAAGTCGGTGGG |
Modification Length | D6 |
Genetic Modification | Deletion |
Location | NA |
Conc. (ng/μl) | 12.5/300 |
Cell line | Embryo |
Method/Assay | T7E1 assay |
Crispr efficiency | 36% |
PRM/crRNA/Vector | T7/pDR274 |
PRM-cas/vector | pMJ806 |
Pubmed | 23360964 |