CrisprGE


     A Central Hub of Crispr based Genome Editing



Crispr2683 details


CrisprIDCrispr2683
OrganismDanio rerio
Gene/Locus (Literature)tph1a
NCBI Gene Nametph1a
Target SequenceCCGGGCTGCGGTTGTGTTTTCCC
Target+Mutant_Flanking

GAGTCTTCAGAGACAGGCCGGGCTGCGGTTGTGTTTTCCCTGAAAAATGAAGTCGGTGGG
GAG - - - - - - - - - - - - -- - - - - - - - - -CGGTTGTGTTTTCCCTGAAAAATGAAGTCGGTGGG

Modification Length D22
Genetic ModificationDeletion
LocationNA
Conc. (ng/μl) 12.5/300
Cell line Embryo
Method/AssayT7E1 assay
Crispr efficiency 36%
PRM/crRNA/VectorT7/pDR274
PRM-cas/vectorpMJ806
Pubmed23360964