CrisprGE


     A Central Hub of Crispr based Genome Editing



Crispr2702 details


CrisprIDCrispr2702
OrganismDanio rerio
Gene/Locus (Literature)gsk3b
NCBI Gene Namegsk3b
Target SequenceGGGACCTGACCGGCCGCAGGAGG
Target+Mutant_Flanking

GTGGTGGCGACTCCTGGACAGGGACCTGACCGGCCGCAGGAGGTCAGCTACACTGACACC
GTGGTGGCGACTCCTGGACAGGGACCTGA
tcctttgtgAGGAGGTCAGCTACACTGACACC

Modification LengthD8,+9
Genetic ModificationIndels
LocationNA
Conc. (ng/μl) 12.5/300
Cell line Embryo
Method/AssayT7E1 assay
Crispr efficiency 27.10%
PRM/crRNA/VectorT7/pDR274
PRM-cas/vectorpMJ806
Pubmed23360964