CrisprGE


     A Central Hub of Crispr based Genome Editing



Crispr2704 details


CrisprIDCrispr2704
OrganismDanio rerio
Gene/Locus (Literature)gsk3b
NCBI Gene Namegsk3b
Target SequenceGGGACCTGACCGGCCGCAGGAGG
Target+Mutant_Flanking

GTGGTGGCGACTCCTGGACAGGGACCTGACCGGCCGCAGGAGGTCAGCTACACTGACACC
GTGGTGGCGACTCCTGGACAGGGACCTGACCGGTCA
- - - - - - - - - - GCTACACTGACACC

Modification LengthD10
Genetic ModificationDeletion
LocationNA
Conc. (ng/μl) 12.5/300
Cell line Embryo
Method/AssayT7E1 assay
Crispr efficiency 27.10%
PRM/crRNA/VectorT7/pDR274
PRM-cas/vectorpMJ806
Pubmed23360964