A Central Hub of Crispr based Genome Editing
CrisprID | Crispr2706 |
Organism | Danio rerio |
Gene/Locus (Literature) | gsk3b |
NCBI Gene Name | gsk3b |
Target Sequence | GGGACCTGACCGGCCGCAGGAGG |
Target+Mutant_Flanking | GTGGTGGCGACTCCTGGACAGGGACCTGACCGGCCGCAGGAGGTCAGCTACACTGACACC |
Modification Length | D13 |
Genetic Modification | Deletion |
Location | NA |
Conc. (ng/μl) | 12.5/300 |
Cell line | Embryo |
Method/Assay | T7E1 assay |
Crispr efficiency | 27.10% |
PRM/crRNA/Vector | T7/pDR274 |
PRM-cas/vector | pMJ806 |
Pubmed | 23360964 |