CrisprGE


     A Central Hub of Crispr based Genome Editing



Crispr2795 details


CrisprIDCrispr2795
OrganismOryzias latipes
Gene/Locus (Literature)DJ-1
NCBI Gene NameDJ-1
Target SequenceCGTCCAGTGCAGCAGAAACGTGG
Target+Mutant_Flanking

AGAGCCCGTCCAGTGCAGCAGAAACGTGGTCATCTGTCCCG
AGAGCCCGTCCAGTGCAGCAG
- - - -- CAGTCATCTGTCCCG

Modification LengthD5
Genetic ModificationDeletion
LocationExon2
Conc. (ng/μl) 25/100
Cell line fertilized eggs
Method/AssaySequencing/PCR
Crispr efficiency 4.3
PRM/crRNA/VectorPDR274
PRM-cas/vectorpCS2+hSpCas9
Pubmed24728957