A Central Hub of Crispr based Genome Editing
CrisprID | Crispr2796 |
Organism | Oryzias latipes |
Gene/Locus (Literature) | DJ-1 |
NCBI Gene Name | DJ-1 |
Target Sequence | CGTCCAGTGCAGCAGAAACGTGG |
Target+Mutant_Flanking | AGAGCCCGTCCAGTGCAGCAGAAACGTGGTCATCTGTCCCG |
Modification Length | D10 |
Genetic Modification | Deletion |
Location | Exon2 |
Conc. (ng/μl) | 25/100 |
Cell line | fertilized eggs |
Method/Assay | Sequencing/PCR |
Crispr efficiency | 4.3 |
PRM/crRNA/Vector | PDR274 |
PRM-cas/vector | pCS2+hSpCas9 |
Pubmed | 24728957 |