A Central Hub of Crispr based Genome Editing
| CrisprID | Crispr2812 |
| Organism | Oryzias latipes |
| Gene/Locus (Literature) | DJ-1 |
| NCBI Gene Name | DJ-1 |
| Target Sequence | TCATCTGTCCCGATACTAGCTTGG |
| Target+Mutant_Flanking | A - - - -- AACGTGGTCATCTGTCCCGATACTA - - - -- - - - - - GCTTGGA |
| Modification Length | 7 |
| Genetic Modification | insertion |
| Location | Exon2 |
| Conc. (ng/μl) | 25/100 |
| Cell line | fertilized eggs |
| Method/Assay | Sequencing/PCR |
| Crispr efficiency | 8.3 |
| PRM/crRNA/Vector | PDR274 |
| PRM-cas/vector | pCS2+hSpCas9 |
| Pubmed | 24728957 |