CrisprGE


     A Central Hub of Crispr based Genome Editing



Crispr2835 details


CrisprIDCrispr2835
OrganismOryza sativa
Gene/Locus (Literature)OsMPK5
NCBI Gene NameMPK5
Target SequenceCCCGTACCTGCTCTACGAC
Target+Mutant_Flanking

GGCCCGTACCTGCTCTACGAC
GGCCCGTA - - - GCTCTACGAC

Modification LengthD3
Genetic ModificationDeletion
LocationNA
Conc. (ng/μl) NA
Cell line Protoplast
Method/AssayPCR, T7E1 assay
Crispr efficiency 4.9
PRM/crRNA/VectorU6/U3
PRM-cas/vectorCaMV35S
Pubmed23956122