A Central Hub of Crispr based Genome Editing
CrisprID | Crispr2841 |
Organism | Oryza sativa |
Gene/Locus (Literature) | OsMPK5 |
NCBI Gene Name | MPK5 |
Target Sequence | ATCGCCACGGAGCTCATGG |
Target+Mutant_Flanking | ATCGCCACGGAGCTCATGGACACCGACC |
Modification Length | D14,+195 |
Genetic Modification | Replacement |
Location | NA |
Conc. (ng/μl) | NA |
Cell line | Protoplast |
Method/Assay | PCR, T7E1 assay |
Crispr efficiency | 10.60% |
PRM/crRNA/Vector | U6/U3 |
PRM-cas/vector | CaMV35S |
Pubmed | 23956122 |