CrisprGE


     A Central Hub of Crispr based Genome Editing



Crispr2841 details


CrisprIDCrispr2841
OrganismOryza sativa
Gene/Locus (Literature)OsMPK5
NCBI Gene NameMPK5
Target SequenceATCGCCACGGAGCTCATGG
Target+Mutant_Flanking

ATCGCCACGGAGCTCATGGACACCGACC
ATCGCC
GGCATCCGCTTCATCACCGACC

Modification LengthD14,+195
Genetic ModificationReplacement
LocationNA
Conc. (ng/μl) NA
Cell line Protoplast
Method/AssayPCR, T7E1 assay
Crispr efficiency 10.60%
PRM/crRNA/VectorU6/U3
PRM-cas/vectorCaMV35S
Pubmed23956122