CrisprGE


     A Central Hub of Crispr based Genome Editing



Crispr2850 details


CrisprIDCrispr2850
OrganismMus musculus
Gene/Locus (Literature)Tet1
NCBI Gene NameTet1
Target SequenceGGCTGCTGTCAGGGAGCTCATGG
Target+Mutant_Flanking

TGGGGCAGGACCAAGTGTGGCTGCTGTCAGGGAGCTCATGGAGACTAGGTGAGGAACTC
TGGGGCAGGACCAAGTGTGGCTGCTGTCAGGGAGCTCA
TGGAGACTAGGTGAGGAACTC

Modification LengthWT
Genetic ModificationNo change
LocationExon4
Conc. (ng/μl) 2.5/2.5
Cell line pronucleus
Method/AssaySequencing/PCR
Crispr efficiency 75.60%
PRM/crRNA/VectorT7
PRM-cas/vectorT7/pCAG
Pubmed24675426