A Central Hub of Crispr based Genome Editing
CrisprID | Crispr2977 |
Organism | Rattus norvegicus |
Gene/Locus (Literature) | Tet1 |
NCBI Gene Name | Tet1 |
Target Sequence | TGGCACCCCAGGCCTATCAGAACCAGGTACTGAAGCCAGGGCT |
Target+Mutant_Flanking | AATAGCTTTCAGGACCTGGCCACTGAACTTGCTCCCTATACAAGCGGCTGGCACCCCAG |
Modification Length | D21,D6 |
Genetic Modification | Deletion |
Location | NA |
Conc. (ng/μl) | 10ng/60 |
Cell line | Cytoplasm |
Method/Assay | Sanger sequencing |
Crispr efficiency | 69.20% |
PRM/crRNA/Vector | pUC19-T7 |
PRM-cas/vector | pEASY-T7 |
Pubmed | 23929337 |