CrisprGE


     A Central Hub of Crispr based Genome Editing



Crispr2984 details


CrisprIDCrispr2984
OrganismRattus norvegicus
Gene/Locus (Literature)Tet1
NCBI Gene NameTet1
Target SequenceTGGCACCCCAGGCCTATCAGAACCAGGTACTGAAGCCAGGGCT
Target+Mutant_Flanking

GTTTGAACACCGAGCTATAGAATGCCG - TTTGGGTCTGAAGGAAGGCCGGCCATTCTCAG
GTTTGAACACCGAGCTATAGAAT - - - -- - - - - - - - - - - - -- - - - - GCCGGCCATTCTCAG

Modification LengthD6
Genetic ModificationDeletion
LocationNA
Conc. (ng/μl) 10ng/60
Cell line Cytoplasm
Method/AssaySanger sequencing
Crispr efficiency 27.70%
PRM/crRNA/VectorpUC19-T7
PRM-cas/vectorpEASY-T7
Pubmed23929337