CrisprGE


     A Central Hub of Crispr based Genome Editing



Crispr3009 details


CrisprIDCrispr3009
OrganismRattus norvegicus
Gene/Locus (Literature)Tet2
NCBI Gene NameTet2
Target SequenceTGGCACCCCAGGCCTATCAGAACCAGG
Target+Mutant_Flanking

CTTGCACCTGATGCCTACAGGAA- - - - - - - - - - - - - - TCAGGTAAGTTGTTGTTCATGAG
- - - - - - - - - - - - -- - - - - - - - - - - 140bp - - - - -- - - - - - CAGGTAAGTTGTTGTTCATGAG

Modification LengthD178
Genetic ModificationDeletion
LocationNA
Conc. (ng/μl) 10ng/60
Cell line Intracytoplasmic sperm injection
Method/AssayPCR/Sequencing
Crispr efficiency NA
PRM/crRNA/VectorpUC19-T7
PRM-cas/vectorpEASY-T7
Pubmed23929337