CrisprGE


     A Central Hub of Crispr based Genome Editing



Crispr3011 details


CrisprIDCrispr3011
OrganismRattus norvegicus
Gene/Locus (Literature)Tet2
NCBI Gene NameTet2
Target SequenceATGAAGACATTGCTGG
Target+Mutant_Flanking

CTTGCACCTGATGCCTACAGGAA- - - - - - - - - - - - - - TCAGGTAAGTTGTTGTTCATGAG
CTTGCACCTGATGCCTACAGG
- - - - - - - - - - - - - - - - - - - - - - AAGTTGTTGTTCATGAG

Modification LengthD7
Genetic ModificationDeletion
LocationNA
Conc. (ng/μl) 10ng/60
Cell line Cytoplasm
Method/AssaySurveyor assay/Sequencing
Crispr efficiency 66.60%
PRM/crRNA/VectorpUC19-T7
PRM-cas/vectorpEASY-T7
Pubmed23929337