CrisprGE


     A Central Hub of Crispr based Genome Editing



Crispr3017 details


CrisprIDCrispr3017
OrganismRattus norvegicus
Gene/Locus (Literature)Tet1
NCBI Gene NameTet1
Target SequenceATGAAGACATTGCTGG
Target+Mutant_Flanking

CTTGCACCTGATGCCTACAGGAA- - - - - - - - - - - - - - TCAGGTAAGTTGTTGTTCATGAG
CTTGCACCTGATGCCTACAGGAA
tcacaatgaagtgaTCAGGTAAGTTGTTGTTCATGAG

Modification Length2
Genetic ModificationInsertion
LocationNA
Conc. (ng/μl) 10ng/60
Cell line Cytoplasm
Method/AssaySurveyor assay/Sequencing
Crispr efficiency 25%
PRM/crRNA/VectorpUC19-T7
PRM-cas/vectorpEASY-T7
Pubmed23929337