CrisprGE


     A Central Hub of Crispr based Genome Editing



Crispr3053 details


CrisprIDCrispr3053
OrganismArabidopsis thaliana
Gene/Locus (Literature)ADH1
NCBI Gene NameADH1
Target SequenceCGTATCTTCGGCCATGAAGCTGG
Target+Mutant_Flanking

TCCCCTATACAAGCGGCTGGCACCCCAGGCCTATCAGAACCAGGTACTGAAGCCAGGGCT
TCCCCTATACAAGCGGC
TGGCACCCCAGGCCTA - - - - - - CCAGGTACTGAAGCCAGGGCT

Modification LengthD14
Genetic ModificationDeletion
LocationNA
Conc. (ng/μl) NA
Cell line Protoplast
Method/AssayHRM,Sanger sequencing
Crispr efficiency 8.00%
PRM/crRNA/VectorU6-26
PRM-cas/vectorPcUbi4-2
Pubmed24836556