CrisprGE


     A Central Hub of Crispr based Genome Editing



Crispr3056 details


CrisprIDCrispr3056
OrganismArabidopsis thaliana
Gene/Locus (Literature)ADH1
NCBI Gene NameADH1
Target SequenceCGTATCTTCGGCCATGAAGCTGG
Target+Mutant_Flanking

TCCCCTATACAAGCGGCTGGCACCCCAGGCCTATCAGAACCAGGTACTGAAGCCAGGGCT
TCCCCTATACAAGCGGC
TGGCACCCCAGGCC - - - - - - - - - - 178bp- - - - - - - - - - - - - - -

Modification Length1
Genetic Modificationinsertion
LocationNA
Conc. (ng/μl) NA
Cell line Protoplast
Method/AssayHRM,Sanger sequencing
Crispr efficiency 8.00%
PRM/crRNA/VectorU6-26
PRM-cas/vectorPcUbi4-2
Pubmed24836556