CrisprGE


     A Central Hub of Crispr based Genome Editing



Crispr3065 details


CrisprIDCrispr3065
OrganismArabidopsis thaliana
Gene/Locus (Literature)ADH1
NCBI Gene NameADH1
Target SequenceCGTATCTTCGGCCATGAAGCTGG
Target+Mutant_Flanking

CTATACAAGCGGCTGGCACCCCAGGCCTATCAGA - ACCAGGTACTGAAGCCAGGGCTTTT
CTATACAAG
- - GCTGGCACCCCAGGCCTATCAG - - - - - - - GTACTGAAGCCAGGGCTTTT

Modification LengthHeteroallelic A/T
Genetic ModificationHeteroallelic A/T
LocationNA
Conc. (ng/μl) NA
Cell line Protoplast
Method/AssayHRM,Sanger sequencing
Crispr efficiency 8.00%
PRM/crRNA/VectorU6-26
PRM-cas/vectorPcUbi4-2
Pubmed24836556