CrisprGE


     A Central Hub of Crispr based Genome Editing



Crispr3322 details


CrisprIDCrispr3322
OrganismSaccharomyces cerevisiae
Gene/Locus (Literature)CAN1.Y
NCBI Gene NameCAN1
Target SequenceGATACGTTCTCTATGGAGGATGG
Target+Mutant_Flanking

GCACTACCTCCGGCGTCGACAT- GCCTGGTGCTG
GCACTACCTCCGGCGTCGACAT
* * ** * * * * *

Modification Length3
Genetic Modificationinsertion
LocationNA
Conc. (ng/μl) 200ng
Cell line Transformation
Method/AssayGalactose induction/PCR
Crispr efficiency 0.07%
PRM/crRNA/VectorSNR52
PRM-cas/vectorGal-L
Pubmed23460208