A Central Hub of Crispr based Genome Editing
| CrisprID | Crispr3354 |
| Organism | Drosophila melanogaster |
| Gene/Locus (Literature) | CG14251 (K81) |
| NCBI Gene Name | k81 |
| Target Sequence | GGATTTCTGATTACGCGGTACGG |
| Target+Mutant_Flanking | AACTTCAGCAGGTGCGGATTTCTGATTACGCGGTACGGGGACCTCAGCGTGTGATTCCCC |
| Modification Length | 6 |
| Genetic Modification | insertion |
| Location | NA |
| Conc. (ng/μl) | 50ng/1ÌÄ_Ìâå_g |
| Cell line | Embryo |
| Method/Assay | PCR |
| Crispr efficiency | 98.90% |
| PRM/crRNA/Vector | T7/pMD19-T gRNA scaffold vector |
| PRM-cas/vector | SP6/pSP6-2sNLS-spcas9 |
| Pubmed | 23833182 |