A Central Hub of Crispr based Genome Editing
| CrisprID | Crispr3454 |
| Organism | Ciona intestinalis |
| Gene/Locus (Literature) | Ebf.774 |
| NCBI Gene Name | Coe |
| Target Sequence | GCTGAGGGTTGGACAACAGGTGG |
| Target+Mutant_Flanking | AAGCAATCAACCAGCTGAGGGTTGGACAACAGGTGGTGGAGCAACAGTTGTTATAGTTGGA |
| Modification Length | NA |
| Genetic Modification | Deletion |
| Location | Exon9 |
| Conc. (ng/μl) | 75/25ÌÄ_Ìâå_g |
| Cell line | Embryo |
| Method/Assay | PCR |
| Crispr efficiency | 46.10% |
| PRM/crRNA/Vector | U6 |
| PRM-cas/vector | nls-Cas9-nls |
| Pubmed | 25336740 |