CrisprGE


     A Central Hub of Crispr based Genome Editing



Crispr3457 details


CrisprIDCrispr3457
OrganismCiona intestinalis
Gene/Locus (Literature)Ebf.774
NCBI Gene NameCoe
Target SequenceGCTGAGGGTTGGACAACAGGTGG
Target+Mutant_Flanking

AAGCAATCAACCAGCTGAGGGTTGGACAACAGGTGGTGGAGCAACAGTTGTTATAGTTGGA
AAGCA - - - - - - - - - - - - - - - - - - -- - - - - - - - - - - - - - - - - -AGCAACAGTTGTTATAGTTGGA

Modification LengthNA
Genetic ModificationDeletion
LocationExon9
Conc. (ng/μl) NA
Cell line Embryo
Method/AssayPCR
Crispr efficiency 46.10%
PRM/crRNA/VectorU6
PRM-cas/vectornls-Cas9-nls
Pubmed25336740