A Central Hub of Crispr based Genome Editing
| CrisprID | Crispr3465 |
| Organism | Danio rerio |
| Gene/Locus (Literature) | eng1bsg5 |
| NCBI Gene Name | eng1 |
| Target Sequence | CCAGTGCCCATCCAGAAACATCC |
| Target+Mutant_Flanking | TCTGTCAAAGCCAGTGCCCATCCAGAAACATCCAGTCC |
| Modification Length | D4,+3 |
| Genetic Modification | Indels |
| Location | NA |
| Conc. (ng/μl) | 6/130 |
| Cell line | Embryo |
| Method/Assay | PCR |
| Crispr efficiency | 50% |
| PRM/crRNA/Vector | PDR274/T7 |
| PRM-cas/vector | PCS2-hspCas9 |
| Pubmed | 25293390 |