CrisprGE


     A Central Hub of Crispr based Genome Editing



Crispr3468 details


CrisprIDCrispr3468
OrganismDanio rerio
Gene/Locus (Literature)eng1bsg5
NCBI Gene Nameeng1
Target SequenceCCAGTGCCCATCCAGAAACATCC
Target+Mutant_Flanking

TCTGTCAAAGCCAGTGCCCATCCAGAAACATCCAGTCC
TCTGTCAAAG
CCAG - - - - - - - - - - -AAACATCCAGTCC

Modification LengthD11
Genetic ModificationDeletion
LocationNA
Conc. (ng/μl) 6/130
Cell line Embryo
Method/AssayPCR
Crispr efficiency 50%
PRM/crRNA/VectorPDR274/T7
PRM-cas/vectorPCS2-hspCas9
Pubmed25293390