CrisprGE


     A Central Hub of Crispr based Genome Editing



Crispr3475 details


CrisprIDCrispr3475
OrganismDanio rerio
Gene/Locus (Literature)vglut2asg2
NCBI Gene NameSlc17a6
Target SequenceGGCTGGCACAGGACTGGCGGCGG
Target+Mutant_Flanking

CTGCTGGCTGGCACAGGACTGGCGGCGGAGCTCGCGCTGATCTGA
CTGCTGGCTGGCACAGGACTGG - - - -- - - - - - - - - - - - - - - - - - A

Modification LengthD22
Genetic ModificationDeletion
LocationNA
Conc. (ng/μl) 6/130
Cell line Embryo
Method/AssayPCR
Crispr efficiency 37.50%
PRM/crRNA/VectorPDR274/T7
PRM-cas/vectorPCS2-hspCas9
Pubmed25293390