A Central Hub of Crispr based Genome Editing
| CrisprID | Crispr3539 |
| Organism | Mus musculus |
| Gene/Locus (Literature) | Rian |
| NCBI Gene Name | Rian |
| Target Sequence | CCATCCATGGTTCACTGAAGGCC . . 23249 . . GGCAAGGTTAGGATTATACAATGG |
| Target+Mutant_Flanking | ATCGTCACCCAGACCATGAAAGG |
| Modification Length | NA |
| Genetic Modification | Deletion |
| Location | NA |
| Conc. (ng/μl) | NA |
| Cell line | Embryo |
| Method/Assay | PCR/T7E1 assay |
| Crispr efficiency | 33% |
| PRM/crRNA/Vector | pUC57 |
| PRM-cas/vector | pST1374-Cas9-N-NLS-flag |
| Pubmed | 25137067 |