A Central Hub of Crispr based Genome Editing
| CrisprID | Crispr3905 |
| Organism | Homo sapiens |
| Gene/Locus (Literature) | miR22 |
| NCBI Gene Name | miR22 |
| Target Sequence | AGAGCTGTGGAGTGTG - - - - - - - - - A - C - AATGG |
| Target+Mutant_Flanking | TTCGTGGCTACAGAGTTTCCTTAGCAGAGCTGTGGAGTGTG - - - - - - - - - A - C - AATGGTGTTTGTGTCTAAA |
| Modification Length | 3 |
| Genetic Modification | Insertion |
| Location | NA |
| Conc. (ng/μl) | 200ng |
| Cell line | HEK293T Cells |
| Method/Assay | T7E1 assay |
| Crispr efficiency | NA |
| PRM/crRNA/Vector | U6, H1 or 7SK |
| PRM-cas/vector | pSSV9-miCMV-Cas9 vector |
| Pubmed | 25186301 |