CrisprGE


     A Central Hub of Crispr based Genome Editing



Crispr3914 details


CrisprIDCrispr3914
OrganismHomo sapiens
Gene/Locus (Literature)miR22
NCBI Gene NamemiR22
Target SequenceGAGTTTCCTTAGCAGAGCTGTGG
Target+Mutant_Flanking

TTCGTGGCTACAGAGTTTCCTTAGCAGAGCTGTGGAGTGTG - - - - - - - - - A- C - AATGGTGTTTGTGTCTAAA
TTCGTGGCTN - - - -- - - - - - - - - - - - - - - - - - TGGAGTGTG - - - - - - -- - A - C - AATGNNNTTTGTGTCTAAA

Modification LengthD22
Genetic ModificationDeletion
LocationNA
Conc. (ng/μl) 200ng
Cell line HEK293T Cells
Method/AssayT7E1 assay
Crispr efficiency NA
PRM/crRNA/VectorU6, H1 or 7SK
PRM-cas/vectorpSSV9-miCMV-Cas9 vector
Pubmed25186301