CrisprGE


     A Central Hub of Crispr based Genome Editing



Crispr3925 details


CrisprIDCrispr3925
OrganismArabidopsis thaliana
Gene/Locus (Literature)FT
NCBI Gene NameFT
Target SequenceAGAGTGATTGATCTATTAAACGG
Target+Mutant_Flanking

AGTAACCTTTAGAGTGATTGATCTATTAAACGGATCAAGAACGTCTCCAACAACTCTGCTT
AGTAACCTTTAGAGTGATTGATCAATTAAACGGATCAAGAACGTCTCCAACAACTCTGCTT

Modification Lengthn.c
Genetic ModificationNA
LocationExon1
Conc. (ng/μl) NA
Cell line Protoplast
Method/AssayT7E1 assay
Crispr efficiency 18.75%
PRM/crRNA/VectorU6p/pGREEN0229
PRM-cas/vectorp3s-Cas9hc
Pubmed25269397