CrisprGE


     A Central Hub of Crispr based Genome Editing



Crispr3936 details


CrisprIDCrispr3936
OrganismArabidopsis thaliana
Gene/Locus (Literature)FT
NCBI Gene NameFT
Target SequenceAAGCCAAGAGTTGAGATTGGTGG
Target+Mutant_Flanking

GGTTCAAAACAAGCCAAGAGTTGAGATTGGTGGAGAAGACCTCAGGAACTTCTATACTTTG
GGTTCAAAACAAGCCAAGAGTTG
- - -- - - - - - - AGAAGACCTCAGGAACTTCTATACTTTG

Modification LengthD10
Genetic ModificationDeletion
LocationExon1
Conc. (ng/μl) NA
Cell line Protoplast
Method/AssayT7E1 assay
Crispr efficiency 84.78%
PRM/crRNA/VectorU6p/pGREEN0229
PRM-cas/vectorp3s-Cas9hc
Pubmed25269397