A Central Hub of Crispr based Genome Editing
| CrisprID | Crispr4177 |
| Organism | Porcine |
| Gene/Locus (Literature) | Oct4gene |
| NCBI Gene Name | POU5F1 |
| Target Sequence | AAGCAGAAGAGGATCACC |
| Target+Mutant_Flanking | GCTCCTAAAGCAGAAGAGGATCACCCTGGGATATACCCAGG |
| Modification Length | NA |
| Genetic Modification | NA |
| Location | Exon2 |
| Conc. (ng/μl) | 10/100 |
| Cell line | Embryo |
| Method/Assay | PCR |
| Crispr efficiency | 37.50% |
| PRM/crRNA/Vector | T7 |
| PRM-cas/vector | pCAG-T3-hCAS-pA |
| Pubmed | 25775469 |