A Central Hub of Crispr based Genome Editing
CrisprID | Crispr4181 |
Organism | Porcine |
Gene/Locus (Literature) | Oct4gene |
NCBI Gene Name | POU5F1 |
Target Sequence | GTATCCTTTCCTCTGCGC |
Target+Mutant_Flanking | GACCAGTATCCTTTCCTCTGCGCCAGGGCCCCATTTTGG |
Modification Length | NA |
Genetic Modification | NA |
Location | Exon5 |
Conc. (ng/μl) | 10/100 |
Cell line | Embryo |
Method/Assay | PCR |
Crispr efficiency | 47.82% |
PRM/crRNA/Vector | T7 |
PRM-cas/vector | pCAG-T3-hCAS-pA |
Pubmed | 25775469 |