CrisprGE


     A Central Hub of Crispr based Genome Editing



Crispr4181 details


CrisprIDCrispr4181
OrganismPorcine
Gene/Locus (Literature)Oct4gene
NCBI Gene NamePOU5F1
Target SequenceGTATCCTTTCCTCTGCGC
Target+Mutant_Flanking

GACCAGTATCCTTTCCTCTGCGCCAGGGCCCCATTTTGG
GACCAGTATCCTTTCCTCTG
- - - -- - - GCCCCATTTTGG

Modification LengthNA
Genetic ModificationNA
LocationExon5
Conc. (ng/μl) 10/100
Cell line Embryo
Method/AssayPCR
Crispr efficiency 47.82%
PRM/crRNA/VectorT7
PRM-cas/vectorpCAG-T3-hCAS-pA
Pubmed25775469