CrisprGE


     A Central Hub of Crispr based Genome Editing



Crispr4190 details


CrisprIDCrispr4190
OrganismHomo sapiens
Gene/Locus (Literature)Pcdh1
NCBI Gene NamePcdh
Target SequenceGCCACACATCCAAGGCTGACAGGGCGGGCACTC . . 1235bp . . TTGAAGATTTGGGGCGTCAGGAAGTGG
Target+Mutant_Flanking

GCAGCCACACATCCAAGGCTGACAGGGCGGGCACTC . . 1235bp . .TTGAAGATTTGGGGCGTCAGGAAGTGGTTTTT
GCAGCCACACATCCAAG - - - -- - - - - - - - - - - - - - - - - - - - - TCCTGCGCGCTGCTCGCCTTCCAAA - - - - - - - - - - TTTT

Modification LengthD1281,+25
Genetic ModificationIndels
LocationNA
Conc. (ng/μl) 2.5/2
Cell line HEK293T Cells
Method/AssayPCR
Crispr efficiency NA
PRM/crRNA/VectorpGL3/U6
PRM-cas/vectorT7
Pubmed25757625