CrisprGE


     A Central Hub of Crispr based Genome Editing



Crispr4252 details


CrisprIDCrispr4252
OrganismHomo sapiens
Gene/Locus (Literature)beta-globin(RE2)
NCBI Gene NameHBB
Target SequenceACCCAATGACCTCAGGCTGTAGGCAGTGG . . . 6,242bp . . . CTACTTTCACTTGTTAGCGGCATCTGTGG
Target+Mutant_Flanking

GCAGACCCAATGACCTCAGGCTGTAGGCAGTGG . . . 6,242bp . . .CTACTTTCACTTGTTAGCGGCATCTGTGGAAGTAG
GC - - - - - - - - - - - - -- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -- - - - - - - - - - - - - - - - - - - - - - - - - - TAG

Modification LengthD6305
Genetic ModificationDeletion
LocationNA
Conc. (ng/μl) NA
Cell line HEK293T Cells
Method/AssayPCR
Crispr efficiency 72.58%
PRM/crRNA/VectorpGL3/U6
PRM-cas/vectorT7
Pubmed25757625