CrisprGE


     A Central Hub of Crispr based Genome Editing



Crispr4258 details


CrisprIDCrispr4258
OrganismHomo sapiens
Gene/Locus (Literature)HoxD
NCBI Gene NameHoxD
Target SequenceGAGGGGTTGGTCTTTGGGAA . . . 18,121bp . . . GAAAGTCATAAACCCGG
Target+Mutant_Flanking

GAGGGGTTGGTCTTTGGGAA . . . 18,121bp . . . GAAAGTCATAAACCCGGGGGATGAAGTGGGAAGACTGTCCAAGGAG
GAGGGGTTGGT - - -- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -- ATGAAGTGGGAAGACTGTCCAAGGAG

Modification LengthD18,151
Genetic ModificationDeletion
LocationNA
Conc. (ng/μl) NA
Cell line HEK293T Cells
Method/AssayPCR
Crispr efficiency 3.57%
PRM/crRNA/VectorpGL3/U6
PRM-cas/vectorT7
Pubmed25757625