CrisprGE


     A Central Hub of Crispr based Genome Editing



Crispr4259 details


CrisprIDCrispr4259
OrganismHomo sapiens
Gene/Locus (Literature)beta-globin
NCBI Gene NameHBB
Target SequenceTAGTATGGCCAGGAGATGGCAGTGTTGA . . . 80,695 bp . . . TCTGGACACCACTAGGGGTCAGAAGTAGTTC
Target+Mutant_Flanking

TAGTATGGCCAGGAGATGGCAGTGTTGA . . . 80,695 bp . . .TCTGGACACCACTAGGGGTCAGAAGTAGTTCATCAAAC
TAGTATGGCCAGGA - - - - - - - - - - - - -- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - GAGGTCAGAAGTAGTTCATCAAAC

Modification LengthD80,723
Genetic ModificationDeletion
LocationNA
Conc. (ng/μl) NA
Cell line HEK293T Cells
Method/AssayPCR
Crispr efficiency 3.57%
PRM/crRNA/VectorpGL3/U6
PRM-cas/vectorT7
Pubmed25757625