CrisprGE


     A Central Hub of Crispr based Genome Editing



Crispr4550 details


CrisprIDCrispr4550
OrganismOryza sativa
Gene/Locus (Literature)MPK5
NCBI Gene NameMPK5
Target SequenceCGGGTACCTGCTCTACGACATCT . . . . . . . . . . . TCTACATCGCCACGGAGCTCATGG
Target+Mutant_Flanking

GCGGCCGGGTACCTGCTCTACGACATCT . . . . . . . . . . . TCTACATCGCCACGGAGCTCATGGACACCGACCT
GCGGCCGGGTA - - - - - - - - - - - -- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - TCATGGACACCGACCT

Modification LengthD727
Genetic ModificationDeletion
LocationNA
Conc. (ng/μl) NA
Cell line Protoplast
Method/AssayPCR/RE
Crispr efficiency 23%
PRM/crRNA/VectorpRGE32/U3 snoRNA
PRM-cas/vectorUBIp
Pubmed25733849