A Central Hub of Crispr based Genome Editing
CrisprID | Crispr4610 |
Organism | Nematostella vectensis |
Gene/Locus (Literature) | NvFP-7R |
NCBI Gene Name | NvFP-7R |
Target Sequence | GGACATTGCTTCGAGATCCAAGG |
Target+Mutant_Flanking | TCAACGGACATTGCTTCGAGATCCAAGGAGTAGGAGAAGGGAAAGCATTC |
Modification Length | D8 |
Genetic Modification | Deletion |
Location | Exon2 |
Conc. (ng/μl) | 700/500 |
Cell line | Unfertilized eggs |
Method/Assay | NA |
Crispr efficiency | 18.70% |
PRM/crRNA/Vector | pDR274 |
PRM-cas/vector | SP6-nlsEGFP-2A |
Pubmed | 25417547 |