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Details of SAPdb entry with Sequence lFF
Primary information
SAPdb ID 1066,
PMID23422591
Peptide NameD-leu-Phe-Phe
Peptide sequencelFF
N-Terminal ModificationFree
C-Terminal ModificationFree
Non-Terminal ModificationNone
Length3
Peptide/ConjuagatePeptide
Conjugate partnerNone
TechniqueCryo - TEM (Transmission Electron Microscopy), AFM (Atomic Force Microscopy), CD (Circular Dichroism spectroscopy), FTIR (Fourier Transform Infrared)
SolventSodium phosphate(0.1M, pH-11.8 and pH5.7)
MethodPeptide (4.0 mg) was added to a solution of sodium phosphate 0.1 M at pH 11.8 (buffer A, 0.3 ml), and dissolution was achieved with sonication (5 min). Gelation was triggered by subsequent addition of an equal volume of 0.1 M sodium phosphate at pH 5.7 (buffer B, 0.3 ml), resulting in a final pH of 7.4.
Conc4mg/ml
TemperatureFinal pH - 7.4
Temperature37°C
Year2013
Self assemblyYes
Type of Self assemblyHydrogel
Tertiary Structure
(Technique)		Not Predicted),
Linear
NA
2 - 3 hours
Hydrogel
NA
None
N[C@H](CC(C)C)C(=O)N[C@@H](Cc1ccccc1)C(=O)N[C@@H](Cc1ccccc1)CO
Primary information
SAPdb ID 1111,
PMID22955637
Peptide NameL-leu-L-Phe-L-Phe
Peptide sequenceLFF
N-Terminal ModificationFree
C-Terminal ModificationFree
Non-Terminal ModificationNone
Length3
Peptide/ConjuagatePeptide
Conjugate partnerNone
TechniqueTEM (Transmission Electron Microscopy), Cryo - TEM (Transmission Electron Microscopy), AFM (Atomic Force Microscopy), CD (Circular Dichroism spectroscopy) and XRD
Solventsodium phosphate buffer
Method4.0 mg of peptide was dissolved in 300 ml of 0.1 M sodium phosphate buffer, pH 11.8 (buffer A), with the aid of sonication for 5 min, then diluted 1 : 1 with another 300 ml of 0.1 M sodium phosphate buffer, pH 5.6–5.7 (buffer B) to yield a final pH of 7.4. Samples were sonicated for another 5 min . Samples left for 24 hours for the formation of gel.
Conc10mg/ml
TemperaturepH 7.4
Temperature25°C
Incubation Time24 hours
Year2012
Self assemblyYes
Type of Self assemblyFibril and Globular Nano-structure
Tertiary Structure
(Technique)		Not Predicted),
Linear
NA
NA
Nanofibers, Nanosphere
150000000
None
N[C@@H](CC(C)C)C(=O)N[C@@H](Cc1ccccc1)C(=O)N[C@@H](Cc1ccccc1)C=O
Primary information
SAPdb ID 1112,
PMID22955637
Peptide NameD- Leu-L-phe-L-phe
Peptide sequencelFF
N-Terminal ModificationFree
C-Terminal ModificationFree
Non-Terminal ModificationNone
Length3
Peptide/ConjuagatePeptide
Conjugate partnerNone
TechniqueTEM (Transmission Electron Microscopy), Cryo - TEM (Transmission Electron Microscopy), AFM (Atomic Force Microscopy), CD (Circular Dichroism spectroscopy) and XRD
Solventsodium phosphate buffer
Method4.0 mg of peptide was dissolved in 300 ml of 0.1 M sodium phosphate buffer, pH 11.8 (buffer A), with the aid of sonication for 5 min, then diluted 1 : 1 with another 300 ml of 0.1 M sodium phosphate buffer, pH 5.6–5.7 (buffer B) to yield a final pH of 7.4. Samples were sonicated for another 5 min . Samples left for 24 hours for the formation of gel.
Conc10mg/ml
TemperaturepH 7.4
Temperature25°C
Incubation Time24 hours- 72 hours
Year2012
Self assemblyYes
Type of Self assemblyHydrogel
Tertiary Structure
(Technique)		Not Predicted),
Linear
NA
Stable
Hydrogel
NA
None
N[C@H](CC(C)C)C(=O)N[C@@H](Cc1ccccc1)C(=O)N[C@@H](Cc1ccccc1)C=O
Primary information
SAPdb ID 1113,
PMID22955637
Peptide NameD- Leu-L-phe-L-phe
Peptide sequencelFF
N-Terminal ModificationFree
C-Terminal ModificationFree
Non-Terminal ModificationNone
Length3
Peptide/ConjuagatePeptide
Conjugate partnerNone
TechniqueTEM (Transmission Electron Microscopy), Cryo - TEM (Transmission Electron Microscopy), AFM (Atomic Force Microscopy), CD (Circular Dichroism spectroscopy) and XRD
Solventsodium phosphate buffer
Method4.0 mg of peptide was dissolved in 300 ml of 0.1 M sodium phosphate buffer, pH 11.8 (buffer A), with the aid of sonication for 5 min, then diluted 1 : 1 with another 300 ml of 0.1 M sodium phosphate buffer, pH 5.6–5.7 (buffer B) to yield a final pH of 7.4. Samples were sonicated for another 5 min . Samples left for 24 hours for the formation of gel.
Conc10mg/ml
TemperaturepH12
Incubation Time24 hours- 72 hours
Year2012
Self assemblyYes
Type of Self assemblyGlobular structure
Tertiary Structure
(Technique)		Not Predicted),
Linear
NA
NA
Others
3000
None
N[C@H](CC(C)C)C(=O)N[C@@H](Cc1ccccc1)C(=O)N[C@@H](Cc1ccccc1)C=O