aspsirna0018 details

Purple = Wild vs mutant target sequence along with mutation information
Brown= Wild vs ASP-siRNA description along with positional differnece between two
Light pink= ASP siRNA efficacy for mutant vs wild allele
Green= Wild siRNA efficacy for Wild vs Mutant allele
siRNA Id:aspsirna0018
siRNA Name: IT-Ds
Gene:Type 1 ryanodine receptor (RyR1)
Disease:Central core disease (CCD)
Wild allele/target (5'->3'): CCGGUGGAGGGAUCGGGGA
Mutant allele/target (5'->3'):CCGGUGGAGGGACCGGUGA
Target Mutation: c.14693T>C,p.Ile4898Thr
Wild siRNA (As strand):GGCCACCUCCCUAGCCCCU
ASP-siRNA (As strand):GGCCACCUCCCUGGCCACU
Mismatch position in siRNA: G7A,A3C
Mutant allele (5'->3'): CCGGUGGAGGGACCGGUGA
ASP-siRNA (As strand):GGCCACCUCCCUGGCCACU
ASP-siRNA efficacy for mutant allele:0
Wild allele (5'->3'): CCGGUGGAGGGAUCGGGGA
ASP-siRNA (As strand):GGCCACCUCCCUGGCCACU
ASP-siRNA efficacy for wild allele:25
Percentage discrimination for ASP-siRNA:-25
Wild allele (5'->3'): CCGGUGGAGGGAUCGGGGA
Wild siRNA (As strand):GGCCACCUCCCUAGCCCCU
Wild sirna efficacy for wild allele:40
Wild allele (5'->3'): CCGGUGGAGGGAUCGGGGA
ASP-siRNA (As strand):GGCCACCUCCCUGGCCACU
Wild sirna efficacy for mutant allele:19
Percentage discrimination for wild siRNA:21
Concentration:200pmol
Orientation:Sense
Mismatch in:siRNA
UniProt ID: P21817
Target sequence: CCGGTGGAGGGACCGGTGA
Genbank Accesion: NM_000540.2
Cell-line: HEK293
Experimental technique used: Confocal microscopy
Transfection method: Lipofectamine 2000
siRNA expression method: Dharmacon
Post-transfection duration: 48 hours
OMID ID: 117000
KEGG disease ID:H00699
Reference:23152933

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