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siRNA Id: | aspsirna0021
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siRNA Name: | YS-Ds
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Gene: | Type 1 ryanodine receptor (RyR1) |
Disease: | Malignanat Hyperthermia (MH) |
Wild allele/target (5'->3'): | UUGUGAACCUGCUCUAUGA
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Mutant allele/target (5'->3'): | UUGUGAACCUGCUCAGCGA
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Target Mutation: | c.1565A>C,p.Tyr522Ser
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Wild siRNA (As strand): | AACACUUGGACGAGAUACU
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ASP-siRNA (As strand): | AACACUUGGACGAGUCGCU
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Mismatch position in siRNA: | C4U,G3A,U5A |
Mutant allele (5'->3'): | UUGUGAACCUGCUCAGCGA
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ASP-siRNA (As strand): | AACACUUGGACGAGUCGCU
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ASP-siRNA efficacy for mutant allele: | 67
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Wild allele (5'->3'): | UUGUGAACCUGCUCUAUGA
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ASP-siRNA (As strand): | AACACUUGGACGAGUCGCU
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ASP-siRNA efficacy for wild allele: | 38
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Percentage discrimination for ASP-siRNA: | 29
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Wild allele (5'->3'): | UUGUGAACCUGCUCUAUGA
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Wild siRNA (As strand): | AACACUUGGACGAGAUACU
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Wild sirna efficacy for wild allele: | 40
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Wild allele (5'->3'): | UUGUGAACCUGCUCUAUGA
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ASP-siRNA (As strand): | AACACUUGGACGAGUCGCU
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Wild sirna efficacy for mutant allele: | 19
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Percentage discrimination for wild siRNA: | 21
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Concentration: | 200pmol |
Orientation: | Sense |
Mismatch in: | siRNA |
UniProt ID: | P21817 |
Target sequence: | TTGTGAACCTGCTCAGCGA |
Genbank Accesion: | NM_000540.2 |
Cell-line: | HEK293 |
Experimental technique used: | RT-PCR |
Transfection method: | Lipofectamine 2000 |
siRNA expression method: | Dharmacon |
Post-transfection duration: | 48 hours |
OMID ID: | 117000 |
KEGG disease ID: | H00699 |
Reference: | 23152933 |