aspsirna0031 details

Purple = Wild vs mutant target sequence along with mutation information
Brown= Wild vs ASP-siRNA description along with positional differnece between two
Light pink= ASP siRNA efficacy for mutant vs wild allele
Green= Wild siRNA efficacy for Wild vs Mutant allele
siRNA Id:aspsirna0031
siRNA Name: YS-Ds
Gene:Type 1 ryanodine receptor (RyR1)
Disease:Malignanat Hyperthermia (MH)
Wild allele/target (5'->3'): UUGUGAACCUGCUCUAUGA
Mutant allele/target (5'->3'):UUGUGAACCUGCUCAGCGA
Target Mutation: c.1565A>C,p.Tyr522Ser
Wild siRNA (As strand):AACACUUGGACGAGAUACU
ASP-siRNA (As strand):AACACUUGGACGAGUCGCU
Mismatch position in siRNA: C4U,G3A,U5A
Mutant allele (5'->3'): UUGUGAACCUGCUCAGCGA
ASP-siRNA (As strand):AACACUUGGACGAGUCGCU
ASP-siRNA efficacy for mutant allele:67
Wild allele (5'->3'): UUGUGAACCUGCUCUAUGA
ASP-siRNA (As strand):AACACUUGGACGAGUCGCU
ASP-siRNA efficacy for wild allele:38
Percentage discrimination for ASP-siRNA:29
Wild allele (5'->3'): UUGUGAACCUGCUCUAUGA
Wild siRNA (As strand):AACACUUGGACGAGAUACU
Wild sirna efficacy for wild allele:40
Wild allele (5'->3'): UUGUGAACCUGCUCUAUGA
ASP-siRNA (As strand):AACACUUGGACGAGUCGCU
Wild sirna efficacy for mutant allele:19
Percentage discrimination for wild siRNA:21
Concentration:200pmol
Orientation:Sense
Mismatch in:siRNA
UniProt ID: P21817
Target sequence: TTGTGAACCTGCTCAGCGA
Genbank Accesion: NM_000540.2
Cell-line: HEK293
Experimental technique used: RT-PCR
Transfection method: Lipofectamine 2000
siRNA expression method: Dharmacon
Post-transfection duration: 48 hours
OMID ID: 117000
KEGG disease ID:H00699
Reference:23152933

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