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siRNA Id: | aspsirna0126
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siRNA Name: | siPrnp178(A9)-6G
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| Gene: | Human prion protein (PRNP) |
| Disease: | Prion disease/Creutzfeldt-Jakob disease |
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Wild allele/target (5'->3'): | UUGUGCACGACUGCGUCAA
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Mutant allele/target (5'->3'): | UUGUGGACAACUGCGUCAA
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Target Mutation: | c.532G>A,p.Asp178Asn
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Wild siRNA (As strand): | AACACGUGCUGACGCAGUU
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ASP-siRNA (As strand): | AACACCUGUUGACGCAGUU
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Mismatch position in siRNA: | C14G,U11C |
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Mutant allele (5'->3'): | UUGUGGACAACUGCGUCAA
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ASP-siRNA (As strand): | AACACCUGUUGACGCAGUU
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ASP-siRNA efficacy for mutant allele: | 52
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Wild allele (5'->3'): | UUGUGCACGACUGCGUCAA
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ASP-siRNA (As strand): | AACACCUGUUGACGCAGUU
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ASP-siRNA efficacy for wild allele: | 88
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Percentage discrimination for ASP-siRNA: | -36
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Wild allele (5'->3'): | UUGUGCACGACUGCGUCAA
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Wild siRNA (As strand): | AACACGUGCUGACGCAGUU
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Wild sirna efficacy for wild allele: | 90
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Wild allele (5'->3'): | UUGUGCACGACUGCGUCAA
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ASP-siRNA (As strand): | AACACCUGUUGACGCAGUU
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Wild sirna efficacy for mutant allele: | 20
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Percentage discrimination for wild siRNA: | 70
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| Concentration: | 40nM |
| Orientation: | Sense |
| Mismatch in: | siRNA |
| UniProt ID: | P04156 |
| Target sequence: | AGTCAGTGGAACAAGCCGAGTAAGCCAAAAACCAACA |
| Genbank Accesion: | NM_000311.3 |
| Cell-line: | HEK293 |
| Experimental technique used: | Western blot |
| Transfection method: | Lipofectamine 2000 |
| siRNA expression method: | Invitrogen |
| Post-transfection duration: | 24 hours |
| OMID ID: | 176640 |
| KEGG disease ID: | H00061 |
| Reference: | 18493311 |
