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siRNA Id: | aspsirna0169
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siRNA Name: | shAPPsw
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| Gene: | Amyloid beta (A4) precursor protein(APP) |
| Disease: | Alzheimers disease |
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Wild allele/target (5'->3'): | UGAAGUGAAGAUGGAUGCAG
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Mutant allele/target (5'->3'): | UGAAGUGAAUCUGGAUGCAG
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Target Mutation: | c.2010_2011delGAinsTC (p.Lys670_Met671delinsAsnLeu)
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Wild siRNA (As strand): | ACUUCACUUCUACCUACGUC
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ASP-siRNA (As strand): | ACUUCACUUAGACCUACGUC
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Mismatch position in siRNA: | G10C,A11U |
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Mutant allele (5'->3'): | UGAAGUGAAUCUGGAUGCAG
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ASP-siRNA (As strand): | ACUUCACUUAGACCUACGUC
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ASP-siRNA efficacy for mutant allele: | 84
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Wild allele (5'->3'): | UGAAGUGAAGAUGGAUGCAG
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ASP-siRNA (As strand): | ACUUCACUUAGACCUACGUC
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ASP-siRNA efficacy for wild allele: | 12
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Percentage discrimination for ASP-siRNA: | 72
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Wild allele (5'->3'): | UGAAGUGAAGAUGGAUGCAG
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Wild siRNA (As strand): | ACUUCACUUCUACCUACGUC
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Wild sirna efficacy for wild allele: | 68
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Wild allele (5'->3'): | UGAAGUGAAGAUGGAUGCAG
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ASP-siRNA (As strand): | ACUUCACUUAGACCUACGUC
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Wild sirna efficacy for mutant allele: | 2
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Percentage discrimination for wild siRNA: | 66
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| Concentration: | 0.01–6.25nM |
| Orientation: | Sense |
| Mismatch in: | siRNA |
| UniProt ID: | P56817 |
| Target sequence: | TCTCTGAAGTGAAGATGGATGCAGAATTCCGAC |
| Genbank Accesion: | NM_000484.3 |
| Cell-line: | Cos-7 |
| Experimental technique used: | Dual luciferase reporter assay |
| Transfection method: | Lipofectamine Plus(invitrogen) |
| siRNA expression method: | Invitrogen |
| Post-transfection duration: | 48 hours |
| OMID ID: | 104300 |
| KEGG disease ID: | H00056 |
| Reference: | 19532137 |
