| |
|---|
|
siRNA Id: | aspsirna0170
|
|
siRNA Name: | shAPPsw
|
| Gene: | Amyloid beta (A4) precursor protein(APP) |
| Disease: | Alzheimers disease |
|
Wild allele/target (5'->3'): | UGAAGUGAAGAUGGAUGCAG
|
|
Mutant allele/target (5'->3'): | UGAAGUGAAUCUGGAUGCAG
|
|
Target Mutation: | c.2010_2011delGAinsTC (p.Lys670_Met671delinsAsnLeu)
|
|
Wild siRNA (As strand): | ACUUCACUUCUACCUACGUC
|
|
ASP-siRNA (As strand): | ACUUCACUUAGACCUACGUC
|
|
Mismatch position in siRNA: | G10C,A11U |
|
Mutant allele (5'->3'): | UGAAGUGAAUCUGGAUGCAG
|
|
ASP-siRNA (As strand): | ACUUCACUUAGACCUACGUC
|
|
ASP-siRNA efficacy for mutant allele: | 60
|
|
Wild allele (5'->3'): | UGAAGUGAAGAUGGAUGCAG
|
|
ASP-siRNA (As strand): | ACUUCACUUAGACCUACGUC
|
|
ASP-siRNA efficacy for wild allele: | 10
|
|
Percentage discrimination for ASP-siRNA: | 50
|
|
Wild allele (5'->3'): | UGAAGUGAAGAUGGAUGCAG
|
|
Wild siRNA (As strand): | ACUUCACUUCUACCUACGUC
|
|
Wild sirna efficacy for wild allele: | 68
|
|
Wild allele (5'->3'): | UGAAGUGAAGAUGGAUGCAG
|
|
ASP-siRNA (As strand): | ACUUCACUUAGACCUACGUC
|
|
Wild sirna efficacy for mutant allele: | 2
|
|
Percentage discrimination for wild siRNA: | 66
|
| Concentration: | 0.01–6.25nM |
| Orientation: | Sense |
| Mismatch in: | siRNA |
| UniProt ID: | P56817 |
| Target sequence: | TCTCTGAAGTGAAGATGGATGCAGAATTCCGAC |
| Genbank Accesion: | NM_000484.3 |
| Cell-line: | Transgenic mouse model |
| Experimental technique used: | allele-specific qPCR |
| Transfection method: | Lipofectamine Plus(invitrogen) |
| siRNA expression method: | Invitrogen |
| Post-transfection duration: | 48 hours |
| OMID ID: | 104300 |
| KEGG disease ID: | H00056 |
| Reference: | 19532137 |
