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siRNA Id: | aspsirna0175
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siRNA Name: | K12-L132P-5
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| Gene: | Keratin 12 (KRT12) |
| Disease: | Meesmann epithelial corneal dystrophy (MECD) |
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Wild allele/target (5'->3'): | AACUAUGCAAAAUCUUAAU
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Mutant allele/target (5'->3'): | AACUAUGCAAAAUCCUAAU
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Target Mutation: | c.409G>C,p.Ala137Pro
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Wild siRNA (As strand): | UUGAUACGUUUUAGAAUUA
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ASP-siRNA (As strand): | UUGAUACGUUUUAGGAUUA
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Mismatch position in siRNA: | G5A |
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Mutant allele (5'->3'): | AACUAUGCAAAAUCCUAAU
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ASP-siRNA (As strand): | UUGAUACGUUUUAGGAUUA
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ASP-siRNA efficacy for mutant allele: | 65
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Wild allele (5'->3'): | AACUAUGCAAAAUCUUAAU
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ASP-siRNA (As strand): | UUGAUACGUUUUAGGAUUA
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ASP-siRNA efficacy for wild allele: | 30
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Percentage discrimination for ASP-siRNA: | 35
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Wild allele (5'->3'): | AACUAUGCAAAAUCUUAAU
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Wild siRNA (As strand): | UUGAUACGUUUUAGAAUUA
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Wild sirna efficacy for wild allele: | 78
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Wild allele (5'->3'): | AACUAUGCAAAAUCUUAAU
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ASP-siRNA (As strand): | UUGAUACGUUUUAGGAUUA
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Wild sirna efficacy for mutant allele: | 24
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Percentage discrimination for wild siRNA: | 54
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| Concentration: | 0.01–6.25nM |
| Orientation: | Sense |
| Mismatch in: | siRNA |
| UniProt ID: | Q99456 |
| Target sequence: | TCTGGATCAGAAAAAGAAACTATGCAAAATCTTAATGATAGAT |
| Genbank Accesion: | NM_000223.3 |
| Cell-line: | Ptk2 |
| Experimental technique used: | Dual luciferase reporter assay |
| Transfection method: | Lipofectamine 2000 |
| siRNA expression method: | MWG Biotech AG |
| Post-transfection duration: | 48 hours |
| OMID ID: | 122100 |
| KEGG disease ID: | H00726 |
| Reference: | 22174841 |
